Lab Manager

Single molecule analysis in live cells

Many different live-cell imaging experiments have been used to study the dynamics of fluorescently tagged proteins.
I am interested in the dynamic nature of Trithorax (TrxG) group proteins, particularly in the TrxG protein absent, small or homeotic disc 1 (ASH1). For quantification of ASH1 residence time on specific binding sites, which are visualized by GFP-tagged polymerase, I am using single molecule tracking (SMT) of a Halo-tagged ASH1 in Drosophila melanogastersalivary glands and wing discs.